Preventive effect of calcium channel blocker in tacrolimus induced nephrotoxicity in rats
10.3760/cma.j.issn.1000-6702.2009.03.004
- VernacularTitle:钙离子通道阻滞剂在预防他克莫司肾毒性中的作用
- Author:
Yehui CHEN
;
Weide ZHONG
;
Yanxiao LIANG
;
Linqiang CHEN
;
Yanmeng LU
;
Jianjian LIANG
;
Jing ZHANG
;
Jiang QIU
;
Weilong LI
;
Keji XIE
;
Jianbo HU
;
Lizhong CHEN
;
Keli ZHENG
- Publication Type:Journal Article
- Keywords:
Tacrolimus;
Nephrotoxicity;
Calcium channel blocker;
Diltiazem
- From:
Chinese Journal of Urology
2009;30(3):156-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the calcium metabolism in tacrolimus(FK506)induced rats nephrotoxicity and the preventive effect of calcium channel blocker.Methods Twenty-four Spragueinduced or FK506-induced nephropathy model.Blood creatinine,blood electrolytes,renal tissue histopathology(HE stain)and the change of ultrastructural organization in renal cells by transmission electron microscope were observed.Results The blood creatinine levels of both CsA and FK506 groups [(36.00±2.61)and(34.17±4.54)μmol/L] were significantly higher than those of the FK506+Dilgroup and control group(all P＜0.05).The blood calcium levels of both CsA and FK506 groups (2.00±0.04 and 2.05±0.04 mmol/L) were significantly lower than those of the FK506+Dil group and control group(all P＜0.05).The blood creatinine and calcium levels of FK506+Dil group were not significantly different with those of control group(P＞O.05).Histopathology examination showed cloudy swelling and vacuolization of the renal tubular epithelial cells and intra-cellular mitochondria swelling and vacuolization in the CsA and FK506 groups.However,the pathological changes of the FK506+Dil group were remarkably milder in comparison with the CsA and FK506 groups.Concluum channel blocker,Dil,could prevent the FK506-induced nephrotoxicity.
