Protection effects of soluble TNFR: Fc fusion protein on acute kidney injury induced by lipopolysaccharide in rats
- VernacularTitle:肿瘤坏死因子受体-IgG1Fc段融合蛋白对脂多糖致大鼠急性肾损伤的保护作用
- Author:
Shiting WANG
;
Zhuying GUO
;
Manghua XU
;
Qiang JIAO
;
Fenghou GAO
- Publication Type:Journal Article
- Keywords:
Receptors,tunmor necrosis factor;
Recombinant fusion proteins;
Kidney disease;
Lipopelysaccharides
- From:
Journal of Chinese Physician
2009;11(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of recombinant human tumor necrosis factor receptor:Fc fusion protein (rhuTNFR:Ice)on the acute renal injury induced by lipepelysaccharide(LPS)in rats.Methods Models ofacute renal injury in rats were constructed by intravenous injection of LPS 10 m/kg.Forty-eight SD rats weighing 180～240g were randomly divided into 4 groups with 12 animals each group,including control group,rhuTNFR:Fc group,LPS groupand rhuTNFR:Fc+LPS group.Mean arterial pressure(MAP)wKs continuously monitored for 6 h.The levels of blood tLrea nitrogen(BUN)。Creatinine(Cr),TNF-αas well as TNF-α bioactivity were assessed.The myeloperoxinse(MPO)and superoxide dismutase(SOD)activity,content ofmalondialdehyde(MDA)were also measured.Pathologic changes of lung tissue in each group were observed by HE staining.Results Compared with LPS group,the status of hypotension and pathological manifestation in kidneys were ameliorated,and MPO activity significantly decreased in rhuTNFR:Fc+LPS group(P<0.05).Conclusion These data suggest that rhuTNFR:Fc can ablate the rise in serum TNF-α bioactivity that occurs in response to LPS,and rhuTNFR:Fc could in part protect rats from the acute renal injury induced by LPS.
