DNT cells isolated and identified in the peripheral blood and its content detection
10.3760/cma.j.issn.1673-4203.2009.03.005
- VernacularTitle:DNT细胞分离鉴定及其在正常人外周血中含量的检测
- Author:
Jiong CHEN
;
Min DU
;
Bing YANG
;
Houkuo TANG
;
Zhimin ZHAI
;
Qing LI
- Publication Type:Journal Article
- Keywords:
DNT cells;
megnetic activated cell sorting;
peripheral blood
- From:
International Journal of Surgery
2009;36(3):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective The immune magnetic separation(MACS)method of DNT cells was set up and their content Was detected in peripheral blood of healthy man.Methods DNT cells were separated from the peripheral blood.Trypan blue staining and flow cytometry were used to detect their activity and purity,and flow cytometry Was used to detect their content in normal human peripheral blood.Results DNT cell activi ty using MACS sorting was＞97%,the purity Was 82.77%;DNT cells accounted for(6.25±2.61)% (n=45)in the TCRαβ+T cells.Conclusion MACS Can quickly sort out high purity DNT cells,and do not affect the vitality of cells.DNT cells accounted for(6.25±2.61)%in the TCRCD3+T cells.
