The effect of ultraviolet ray exposure on DNA methylation in systemic lupus erythematosus

VernacularTitle:紫外线暴露对系统性红斑狼疮患者DNA甲基化水平的影响
Author: Min ZHANG ; Guosheng WANG ; Xiangpei LI ; Wei CHEN ; Ming KAN ; Yunmin WANG ; Xiaodong HE
Publication Type:Journal Article
Keywords: Lupus erythematosus,systemic; Ultraviolet rays; DNA methylation; DNA methyhmnsferase 1
From: Chinese Journal of Rheumatology 2009;13(3):188-192
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of ultraviolet ray on DNA methylation in systemic lupus erythematosus (SLE) and its role in the pathogenesis of SLE.Methods Peripheral blood mononuclear cells (PBMC) were isolated from 45 SLE patients and 20 healthy controls and were cultured in vitro.Then they were irradiated with 311 nm narrow band-ultraviolet my (NB-UVB) in various dosages.DNA methylation was,detected by high-performance capillary electrophoresis and DNA methyltransferase 1 (DNMT1) mRNA expression was measured by real-time quantitative PCR.Results The level of DNA methylation in SLE pafients was lower than that in the control group (P=0.014).DNA methylation was,decreased after UVB exposure with the dosage of 50,100 mJ/cm2 (P＜0.01),but no significant difference could be found in the DNMT1 mRNA expression.No significant correlation was found between the level of DNA methylation and systemic lupus erythematosus activity index (SLEDAI).Conclusion Changes take palce in DNA methylation after UVB exposure,and UVB may affect the pathogenesis of SLE by changing DNA methylation.