Association between single nucleotide polymorphisms of thrombospondin-1 gene and coronary artery disease in the Chinese Han population

VernacularTitle:中国汉族人血小板反应素蛋白1基因单核苷酸多态性与冠心病的关联研究
Author: Jian DAI ; Haiming LUO ; Zhijian YANG ; Zhonglong DENG ; Xunjie ZHOU ; Wang YAO ; Jinan ZHANG
Publication Type:Journal Article
Keywords: Coronary artery disease; Thrombospondin-1 gene; Single nucleotide potymorphisms; Polymerase chain reaction and restriction fragment length polymorphism; DNA sequencing
From: Clinical Medicine of China 2009;25(3):247-249
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between single nucleotide polymorphisms(SNPs)of thrombospondin-1 gene(TSP-1)and coronary artery disease(CAD).Methods Fragment 8831A→G of Exon thirteen in TSP-1 gene from 437 cases of CAD and 423 subjects without coronary heart disease from November 2003 to May 2007 in The First Affiliated Hospital of Nanjing Medical University and Affiliated Yueyang Hospital of Shanghai Traditional Chinese Medicine University were analysed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP),and sequence analysis for confirmation.Results The prevalence of the 8831G in the 860 subjects was rare.No association of the N700S polymorphism with an altered risk of ACS was found in our study (GA VS AA:OR=1.699;95%CI 0.309-9.348,P＞0.05).Conclusion The TSP-1 8831A→G polymorphism is rare and unrelated to CAD in the Chinese Han population.