Effects of wt-p53, BT-1 and CM-CSF genes cotransfection on proliferation and immunogenicity of SH-SY5Y cell lines
10.3760/cma.j.issn.1673-4408.2009.02.003
- VernacularTitle:多重基因转染对神经母细胞瘤SH-SY5Y细胞增殖和免疫原性的影响
- Author:
Fang CHEN
;
Lei LIU
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Neuroblastoma;
Adenoviruses,Human;
Genes,p53;
Antigens,CD80;
Granulocyte-macrophage colony-stimulating factor
- From:
International Journal of Pediatrics
2009;36(2):116-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effeet of cotransduction of wt-p53,B7-1 and GM-CSF genes mediated by recombinant adenovirus on cell growth, apoptosis and immunogenicity of neuroblastoma cell line SH-SY5Y, Methods We transfected SH-SYSY cells separately with recombinant adenovirus, recombinant adenovirus mediating human wild type p53(wt-p53) gene and BB-102.We separately named them with SH/Ad,SH/p53 and SH/BB-102 group.The protein expression of p53, B7-1 and GM-CSF were measured by Western blot, FCM and enzyme linked immunosorbent assay. Cell number was counted and growth curves were drawed. Cell apoptosis was tested by FCM. Lymphocyte proliferation and cytokine secretory was evaluated by mixed lymphocyte culturing. Results After transection with BB- 102, p53, B7-1, and GM-CSF, high-efficiency expression of target genes were found. Growth of the cells was inhibited, apoptosis was induced in the SH/p53 and SH/BB-102 groups. Proliferation of lymphocytes was stimulated and cytokine increased visorously in the SH/BB-102 group. Condusions After transfection with BB-102, growth of the SH-SY5Y cells was inhibited,apoptosis was induced,and immunogenicity was greatly enhanced.
