Effect of OB-cadherin gene transfection on osteogeule differentiation of human mesenchymal stem cells in vitro

VernacularTitle:成骨细胞特异性钙黏蛋白基因转染对人骨髓基质干细胞成骨分化影响的研究
Author: Qiang XIANG ; Congying DENG ; Wenjie ZHENG ; Guoning GUO ; Yuan ZHANG ; Chao ZHANG ; Yue ZHOU
Publication Type:Journal Article
Keywords: Cadherins; Cad- Ⅱ; Mesenchymal stem cells; Osteogenic differentiation; Scaffolds
From: Chinese Journal of Orthopaedic Trauma 2009;11(3):259-262
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Cad-Ⅱgene on osteogenic differentiation of human mesenchymal stem cells in vitro. Methods The human marrow mesenchymal stem cells (hBMSCs) were isolated and cultured in vitro before they were divided into 2 groups. In the transfeetion group, the hBMSCs were transfeeted with Cad- Ⅱgene;in the simple osteogenic inducement group, they were cultured in the condition medium. Then the expressions of Cad- Ⅱ protein were determined and the alkaline phosphatase (ALP) activities and the expressions of ostcocalein were measured at 3, 7, 14 and 21 days for comparison between the 2 groups. Results The ALP activity and positive expression of osteoealcin were regulated significantly higher in the transfeetion group than in the simple osteogenic inducement group at different times (P <0.05). The mineralized nodes began to appear at 14 days in the 2 groups and increased with time. Conclusion Cad-Ⅱgene transfeetion can promote differentiation of hBMSCs into the osteoblasts.