An experimental study on improving quality of routine cryopreserved islets cultured with hyperbaric RCCS and transplantation
10.3760/cma.j.issn.1007-631X.2009.03.017
- VernacularTitle:高压氧微重力培养提高冻存胰岛质量和移植效果的实验研究
- Author:
Yi ZHOU
;
Rui LIU
;
Jianyu WU
;
Wenjie DAI
;
Chunfang SONG
- Publication Type:Journal Article
- Keywords:
Islet of Langerhans transplantation;
Hyperbaric oxygenation;
Rats,wistar;
Cryopreservation;
Microgravity;
Rotary cell culture system
- From:
Chinese Journal of General Surgery
2009;24(3):221-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of frozen-thawed murine islets which were transplanted into diabetic rats after cultured with hyperbaric oxygenated rotary cell culture system (HORCCS). Methods The purified rat islets were divided into two groups: A. In vitro experiment groups (IvEG) : The rat islets in each subgroup were cultured in HORCCS or common medium for 30 days, then evaluated for the intracellular DNA and insulin contents of islets, and the viability and insulin secreting level of islets. B. Islet transplantation experimental groups (TxEG) : The frozen-thawed islets were cultured in HORCCS or common medium for 7 days, and then transplanted into the recipients. We observed the blood glucose level (BGL) and insulin secreting level in the recipients as well as the uhrastructure change of islets in TxEG. Results The viability and insulin secreting level of islets cultured with HORCCS at 14th day were much higher than those cultured with common medium (P <0.05). The blood glucose level in recipients transplanted with islets cultured with HORCCS recovered to normal value at the 2nd week and lasted for 8 weeks. All these recipients maintained the normal glucose tolerance curve. Electronic microscopy found microchannel outlets on the surface of the frozen-thawed islets cultured with HORCCS. Conclusions Frozen-thawed islets cultured with HORCCS could establish nutrient transmission microchannels, which were not only capable of oxygen and nutrients transmission, but also improving cryopreservation solution to diffuse inside the islet cells evenly and uniformly. So this method not only lessens islet damage from cryopreservation, but also improves the effect of transplantation.
