Culture and identification of pig retinal pigment epithelial cells in vitro
- VernacularTitle:猪视网膜色素上皮细胞的体外培养和鉴定
- Author:
Lin LIN
;
Yazhen WU
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye/CY;
Cell culture techniques;
Swine
- From:
Journal of Chinese Physician
2009;11(2):202-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.
