Experimental study on thrombopoietin providing protection against adriamycin-induced oxidative damage of myocardial cell in rats
- VernacularTitle:血小板生成素抑制阿霉素大鼠心肌细胞氧化损伤的实验研究
- Author:
Feiheng CHEN
;
Yuansheng LIU
;
Yongzhong SU
;
Xishui CHEN
;
Meiluan JIANG
;
Huijun LI
;
Fengjiao TAN
;
Zhanwen HOU
- Publication Type:Journal Article
- Keywords:
Thrombopoietin/PD;
Cardiomyopathies;
Myocytes,cardiac/DE;
Oxidative stress
- From:
Journal of Chinese Physician
2009;11(1):73-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inwst the antagonistic effect of thrombopoietin on adriamycin induced myocardium injury in rats and explore the mechanism.Methods 32 Wistar rats were randomized into four groups(n=8):Control group,ADM group,ADM+TPOL group and ADM+TPOH group.All agents were given by intraperitoneal injection.The control group was given saline.While the other three groups were given adriamycin at the dosage of20mg/kg.TPO group were injected TPO at the dosages of 10μg/kg or 30μg/kg three times on alternale days.ELISA was used to detect the concentration of CK-MB and cTnI in the serum of rats.The change of cardiocyte ultrastructure was observed by the electron microscope,and pathological change Was observed by immunohistochemistry staining.The expression level of 8- hydroxy-2'-deoxyguanosin(8-OHdG)produced by DNA oxidative damage in myocard tissue were detected.IPP6.0 software was used to detect IOD and calculate the 8-OHdG index.Results The energy of CK-MB and cTNI of TPO group was obviously lower than that in ADM group(P<0.01).The ultragtrueture of cardiocyte in the ADM group Wag damaged more severely than that in TPO group.Pathological Score,IOD and 8-OHdG index of TPO groups were lower than ADM group(P<0.05).These indexes had no significant statistics difference between ADM+TPOL group and ADM+TPOH group.Conclusions TPO can provide heart protection by antagonizing oxidative damage of myocardial cell induced by edriamycin.
