The construction of bioartificial liver by BMSC and alginate scaffold
10.3760/cma.j.issn.1007-631X.2009.03.020
- VernacularTitle:以海藻酸三维支架构建骨髓间充质干细胞源性生物人工肝组织的实验研究
- Author:
Jizong LIN
;
Zhaofeng TANG
;
Heping FANG
;
Nan LIN
;
Kunpeng HU
;
Jun YANG
;
Peng XIANG
;
Ruiyun XU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Liver,artificial;
Cell culture techniques;
Alginatescaffold
- From:
Chinese Journal of General Surgery
2009;24(3):234-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the bioartificial liver by bone mesenchymal stem cell (BMSC) and alginate scaffold. Method Alginate scaffold was used as the cell carrier for the cultivation of BMSC and the differentiation from BMSC into hepatic like cells was induced by the cell factors of HGF, EGF and FGF-4 in the scaffold in vitro. The compatibility of the cells and the scaffold was observed by microscopy and the function of the differentiatd cells was tested. The gene of AFP and ALB was detected by RT-PCR. The secretion of ALB and the urea synthesis of the cells were tested by ALB kit and urea kit respectively. The glycogen synthesis and the CK-18 was tested by the glycogen stanning method and the immunofluorescence test. Results BMSC was able to attach, grow and proliferate well in the alginate scaffold, the well compatibility was observed by microscopy. ALB and urea were detected in the cultivating medium, the gene of ALB and AFP was identified by RT-PCR. The glycogen synthesis ability and the expression of CK-18 were induced during the differentiation. Conclusion The three dimensional atginate scaffold exhibited well compatibility with BMSC, BMSC could be differentiated into the hepatic like cell in the scaffold. BMSC and the alginate scaffold could be used to construct the bioartificial liver for the hepatic tissue engineering.
