Effect of losartan on transforming growth factor β1 in alcoholic nephropathy in rats
- VernacularTitle:洛沙坦对酒精性肾损伤大鼠肾脏转化生长因子β1表达的影响
- Author:
Yundou WU
;
Ying CHEN
- Publication Type:Journal Article
- Keywords:
Losartan/PD;
Alcoholism/CO;
Kidney disease;
Transforming growth factor beta
- From:
Journal of Chinese Physician
2009;11(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of losartan,an angiotensin 1I type 1 receptor antagonist(ATlRA),on transforming growth factor-β1 expression in alcoholic nephropathy in rats.Methods Male adult Wistar rats were random divided into three subgroups and treated daily for 12 weeks as follows,rats were treated with normal saline[20ml/(kg·d)gavage twicely]in vehicle group,rats were treatedwith alcohol[400g/L,8g/(kg·d)gavage twieely]in alcoholic nephropathy,and rats were concomitantly treated with alcohol and Iosartan [200mg/L,ling/(kg·d),gavage]in alcohol+losartan.HE staining,Sirius red staining,electronic microscope,and immunohistochemistry were used to examine histologic changes,collagen fibers deposition,and the expression of TGF-BI.Results Compared with vehicle treated rats,alcoholic nephropathy rats showed tubular vacuolation,granular degeneration,swelling,tubulointerstitial inflammatory cell infil tration,and rarely mesangial proliferation and matrix deposition.Sirius red staining displayed a significant increase in collagen fibers in alias of glomemli,tubulointerstitium and vessels.Concomitant administration of losartan reversed all of above parameters(P<0.05).The upregulated expression of TGF-β1 proteins was significantly suppressed by losartan treatment(P<0.05).Conclusions These findings suggest that alcoholic nephropathy is characterized by the tubular granular degeneration,vacuolation,swelling,and tubulointemtitial inflammato-ry cell infiltration,and this is associated with upregulation of TGF-β1.Losartan treatment offers protection effect by downregulating TGF-β1 expression.
