Hydrodynamics-based transfection of IL-10 and TGF-β1 plasmids prolonging the graft survival in murine skin transplant model
10.3760/cma.j.issn.0254-1785.2009.03.004
- VernacularTitle:体内注射白细胞介素10和转化生长因子质粒延长小鼠移植皮肤存活时间
- Author:
Peng XIA
;
Yirong YANG
;
Wenwei CHEN
;
Shaoling ZHENG
;
Yong CAI
;
Longren XIAO
;
Bicheng CHEN
- Publication Type:Journal Article
- Keywords:
Interleukin-10;
Transforming growth factor beta;
Skin transplantation;
Graft survival
- From:
Chinese Journal of Organ Transplantation
2009;30(3):144-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hydrodynamics-based injection with plasmids of IL-10, TGF-β1 and TGF-β1 + IL-10 in murine skin transplantation model. Methods Plasmids were constructed by inserting coding sequences of IL-10 and TGF-β1. In F1 mice (Balb/c×C57BL/6, H-2b/d) to Balb/c (H-2d) murine skin transplant model, 20 μg plasmid (blank or IL-10 or TGF-β1 or IL-10 + TGF-β1) was injected to donors by hydrodynamics-based method in first day and every interval 2 days for 6 times. The survival of grafts was observed after 7 days of transplantation. After C56BL/6 spleen cells transfused Balb/c accepted 5 times hydrodynamics-based injection as above,CD4+ CD25+ T regulatory cells of spleen were measured by FACS. Results The survival time of graft in each group was (13.50±1.04)days (blank group), (13.83±1.16)days (IL-10 group), (15.33±1.50) days (TGF-β1 group), and (21.33±3.20) days (IL-10 + TGF-β1 group),respectively (P<0.05). The percentage of CD4+ CD25+ cells was (6. 58±1.86)% (blank group),(10.52±1.13)% (IL-10 group),(14.44±0.42)% (TGF-β1 group),and (14.25±1.24)% (IL-10+TGF-β1 group) respectively (P<0.05). Conclusion Hydrodynamics-based transfection of IL-10 combined with TGF-β1 can synergistically enhance the percentage of CD4+ CD25+ T cells and prolong the graft survival.
