D-limonene inhibits the proliferation of human bladder cancer cells and down-regulates the ras p21 expression
- VernacularTitle:D-柠檬烯对人膀胱癌细胞生长和ras p21表达的影响
- Author:
Yaoting XU
;
Wei GU
;
Jiangxia LIU
;
Ruizi LIU
;
Dujian LI
;
Ruqiang HUANG
;
Xiaowen XU
;
Min XIE
- Publication Type:Journal Article
- Keywords:
D-limonene;
Bladder neoplasms;
Carcinoma;
Genes
- From:
Chinese Journal of Urology
2008;29(12):808-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of D-limonene on the proliferation and ras P21 expression in human bladder carcinoma EJ cell.Methods The cell proliferation was measured by XTT assay.The expression of ras mRNA was detected by real-time PCR,and the expression of its protein product was analyzed by Western Blot.Results The proliferation of EJ cell was inhibited by exposure to 2 mmol/L and 4 mmol/L D-limonene for 48h.The optical density (A) was 0.801±0.016 and 0.148±0.008,respectively.There were significant differences between the experimental groups and the control group (1.2181±0.031,P<0.05).The expressions of ras P21 and its protein product of EJ cell were decreased obviously when exposure to 1 mmol/L of D-limonene for 48h.The expres-sions were significantly different compared with the control group(P<0.05).Conclusions D-limo-nene could inhibit human bladder carcinoma EJ cell proliferation,which is in a dose-dependant man-ner.In a lower dose (<1 mmol/L),D-limonene can decrease the expression of ras mRNA and its protein product.
