Expression of Snail and tumor invasiveness in breast carcionma

VernacularTitle:乳腺癌组织中Snail蛋白表达与肿瘤侵袭力的研究
Author: Ali ZHANG ; Quansheng WANG ; Yahua ZHONG ; Gang CHEN ; Ling XI ; Conghua XIE ; Yunfeng ZHOU ; Ding MA
Publication Type:Journal Article
Keywords: Breast neoplasms; Snail
From: Chinese Journal of General Practitioners 2009;8(4):264-266
CountryChina
Language:Chinese
Abstract: Immunohistochemistry was used to detect the protein expression of Snail in 5 specimens jusxta-eancerous normal breast tissues, 35 specimens of cancerous tissues without metastasis and 20 specimens of breast carcinoma with lymphonode metastasis. Breast carcinoma cell line MDA-MB-231 was transfected with antisense Snail. Results showed that the expression of Snail protein was significantly higher in breast carcinomas than in their normal tissues. The mRNA and protein expressions of Snail in the breast carcinoma cells treated with antisense Snail was significantly decreased while the E-cadherin protein significantly increased (both P < 0.05). The number of invasive ceils treated with antisense Snail was (10.5±1.3)%, while in treated with EGFP was (68.2±2.1)% (P < 0.05). The abnormal expression of Snail contribute to the invasiveness of breast carcinoma. The antisense Snail could prevent the cells ability to invade in vitro, and the effect is related with the up-regulated E-cadherin protein.