The expression of DJ-1 protein in proteomic analysis of late-onset Alzheimer disease
10.3760/cma.j.issn.0578-1426.2009.04.006
- VernacularTitle:DJ-1蛋白在晚发型阿尔茨海默病蛋白组学的表达
- Author:
Xianhong CHEN
;
Luning WANG
;
Hongli WANG
;
Bingyu LIU
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Proteomics;
DJ-1 protein
- From:
Chinese Journal of Internal Medicine
2009;48(4):277-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathogenicity of late-onset Alzheimer disease from the viewpoint of comparative proteomic technology and to screen it from diseases with related protein markers.Methods Cerebral cortex tissue of temporal layer of 8 cases of late-onset Alzheimer disease and 5 cases of age-matched autopsied controls with normal brain was chosen for this study.Cerebral proteins were run through immobilized pH gradient (IPG) isoelectric focusing electrophoresis as the first dimension and then vertical SDS-PAGE electrophoresis as the second dimension.Differential proteins were identified with visionworks LS and then analyzed with matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS) and eleetrospray ionization mass spectrometry (ESI-MS).Finally,the protein was identified by searching in the data bank.Results Different 2-dimensional gel electrophoresis maps were obtained for the protein spots in the late-onset Alzheimer disease group and the control group gels.11 protein spots showed a significantly differential expression between the two groups of cerebral cortex samples.It was found that the expression of DJ-1 protein was increased in the late-onset Alzheimer disease group in comparison with the control group after searching in the database.Conclusion DJ-1 protein may be a potential marker related to Alzheimer disease pathogenicity.This finding would be helpful to develop new drugs which focus on this protein and prevent nearodegeneration.
