Expression of nerve growth factor receptors p75 in human bladder carcinoma and its influence by hypoxia
10.3760/cma.j.issn.1000 6702.2009.04.016
- VernacularTitle:神经生长因子受体p75在膀胱癌组织和缺氧条件下膀胱癌T24细胞株中的表达
- Author:
Changhui FAN
;
Jianguo WEN
;
Baoping QIAO
;
Zhenhe SUO
;
Zhiqiang ZHU
;
Qingwei WANG
- Publication Type:Journal Article
- Keywords:
Nerve growth factor receptors;
Bladder neoplasms;
Carcinoma;
Hypoxia
- From:
Chinese Journal of Urology
2009;30(4):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of the nerve growth factor receptors p75 (p75NGFR) in human bladder carcinoma samples, and the effects of hypoxia on the expression of p75NGFR in human bladder cancer ceils. Methods The expression of p75NGFR in 107 bladder cancer and lymph node specimens were immunohistochemically investigated. The expression of p75NGFR in bladder cancer cell line (T24) was assessed by immunocytochemistry, and reverse tran-scription-PCR in the Normoxic Condition (air, 5%CO2) and in hypoxia condition(10%O2, 5%CO2, 85%N2). Results p75NGFR expressed in 46 of 107(43.0%) tumor samples. There was no signifi-cant correlation between p75NGFR and the factors such as gender, age, extent of the tumors, and pathologic grading(P0.05), p75NGFR was expressed in examined cell line T24, and also expressed in 5 of 24 bladder tumors in metastasized lymph node specimens. Hypoxia markedly down-regulated the expression of p75NGFR of T24 cell line at third day. Conclusions It is suggested that p75NGFR is expressed less in lymph node metastasis. Hypoxia markedly inhibited expression of p75NGFR of T24 cell lines.
