Study on the effects of acidosis on transient receptor potential channel M7 (TRPM7) in mouse cardiac fibroblast
10.3760/cma.j.issn.0254-9026.2009.04.022
- VernacularTitle:酸中毒对小鼠成纤维细胞瞬时受体电位通道亚族M7影响的实验研究
- Author:
Yongming ZHOU
;
Mingjiang LI
;
Tianyi QIU
;
Yanhong TANG
;
Congxin HUANG
- Publication Type:Journal Article
- Keywords:
Fibroblasts;
Myocardium;
TRPM cation channels;
Acidosis
- From:
Chinese Journal of Geriatrics
2009;28(4):320-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of acidosis on the current change of transient receptor potential channel M7 (TRPM7) and collagen production in mouse cardiac fibroblast (MCFs), and to explore the pathophysiological function of TRPM7 on the cardiac fibrosis. Methods (1) The model of MCFs was established and isolated. (2) MCFs was subcuhured. (3) Patch clamp technique was used to observe the current characteristics of TRPM7 in low PH solutions. (4) The influence of acidic condition on Ca2+ influx in MCFs was recorded by calcium fluorescent indicators. Results (1) There was a high level expression of TRPM7 in MCFs and the electrophysiological characteristics of TRPM7-like (TRPM7L) was similar to that of TRPM7. (2) Ca2+ influx was increased in acidic condition, and the F340/F380 ratio was increased from 1.0 to 4. 6 at pH 4.0 compared with pH 7.0 (t=2.72, P<0.01). Conclusions (1) TRPM7 is the molecular basis of the native TRPM7L in MCFs and TRPM7L plays an important role in Ca2+ influx. (2) The pathophysiological function of MCFs is influenced by regulation of Ca2+ influx mediated by TRPM7L in the condition of acidosis.
