Effects ot VEGF-165 gene therapy on apoptosis in brain traumatic injury
10.3760/cma.j.issn.1671-0282.2009.04.008
- VernacularTitle:血管内皮细胞生长因子165基因对创伤性脑损伤细胞凋亡的影响
- Author:
Ke LIU
;
Wenyuan TANG
- Publication Type:Journal Article
- Keywords:
Vascular endthelial growth factor(VEGF);
Traumatic brain injury(TBI);
Apoptosis;
Gene therapy
- From:
Chinese Journal of Emergency Medicine
2009;18(4):367-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of apoptosis in brain after traumatic injury(TBI)treated with exogenous vascular endothelial growth factor(VEGF)gene therapy in oder to find out the role of exogenous VEGF gene in protectiog brain tissue.Method The injurea cerebral cortex from the rat models of brain with traumatic injury was injected with adenovirus(adenovirus,Ad)as the carrier of VEGF-165 gene(Ad-VEGF-165 Gene).RT-PCR and Western blot were used to detect the expression of VEGF mRNA and VEGF protein in the brain 6 h,24 h,3 d,7 d and 14 d after injury,and apoptosis in the injured location of brain was also detected by TUNEL successively after Ad-VECF-165 gene therapy.Results With exogenous Ad-VEGF-165 applied to the locally injured brain tissue after injury,VEGF mRNA and VEGF protein showed consistent expression and their expressions were significantly higher than those in trauma group and vehicle control group.Compared with the trauma group.the apoptosis in the gene therapy group 24 h,3 d and 7 d after injury presented a.significant reduction,and had close relationship with VEGF.(Control group:4.17±0.73;TBI group,24 h:47.18±6.01,3 d:79.44±11.23;TBI+VEGF group.24 h:28.72±5.31,3 d:54.18±7.66;P＜0.05).Conclusions The exogenous VEGF gene therapy administered to have protective effects on the local brain tissue in rats with traumatic injury in a certain time.
