Abnormal expressions of Nav1.1 and Nav1.2 in hippocampus following traumatic brain injury in rats
10.3760/cma.j.issn.1001-8050.2009.04.95
- VernacularTitle:颅脑创伤后大鼠海马Nav 1.1和Nav 1.2的异常表达
- Author:
Qing MAO
;
Feng JIA
;
Yongming QIU
;
Xiaohua ZHANG
;
Jianwei GE
;
Fengping YU
;
Qizhong LUO
;
Jiyao JIANG
- Publication Type:Journal Article
- Keywords:
Brain injuries;
Sodium channels;
Hippocampus;
Neuroprotection
- From:
Chinese Journal of Trauma
2009;25(4):309-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA and protein expressions of Nav 1.1 and Nav 1.2 in hippocampus following traumatic brain injury ( TBI) in rats.Methods After the lateral fluid percussion model was established in adult male Sprague Dawley rats,the rats were sacrificed at 2,12,24 and 72 hours after percussion and collected ipsilateral hippocampus for detecting mRNA and protein expressions of Nav 1.1 and Nav 1.2 by means of fluorescent quantitation RT-PCR,Western blot and immunofluo rescence staining.Results The mRNA expressions of Nav 1.1 and Nav 1.2 were significantly down-regulated (P<0.01) in hippocampus and reached the lowest level at 2 hours following TBI.The protein expression of Nav 1.1 was significantly down-regulated (P<0.01) but recovered near to level of control group at 72 hours after TBI.While there was no statistical difference on protein expression of Nav 1.2 in hippocampus after TBI compared with control group (P>0.05).Conclusion TBI induces significant down-regulated mRNA and protein expressions of Nav 1.1 in the hippocampus,which may be one of molecular mechanisms for functional alternation of sodium channels and excitotoxic action following TBI.
