Tat-NBD peptide inhibits inflammation effects related to NF-kB on AR42J cell
10.3760/cma.j.issn.1671-0282.2009.04.018
- VernacularTitle:Tat-NBD多肽抑制胰腺腺泡细胞AR42J细胞的核因子-kB相关性炎症反应
- Author:
Youming LONG
;
Xuejin LIU
;
Ken CHEN
;
Wenrui XIE
;
Hui WANG
- Publication Type:Journal Article
- Keywords:
Acute pancreatitis;
Lipopolysaecharide;
Nuclear factor-kappa B;
Tumor necrosis factor;
Peptide;
Pancreatic acinus AR42J cell
- From:
Chinese Journal of Emergency Medicine
2009;18(4):401-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tat-NBD(NEMO-binding domain,NBD)peptide on LPS-stimulated AR42J acinus cells inflammatory response.Method Lipopolysaccharide(LPS)was added to culture media at doses of 10 mg/kg for 24 hours to stimulate the AR42J cells.For pretreatment.cells were incubated with different peptides for 2 hours before LPS stimulation.The expression of TNF-α mRNA WaS conducted using a semi-quantitative RT-PCR method.TNF-α protein in culture medium were detected by enzyme linked immunosorbent assay(ELISA).The expression and translocation of the NF-kB-p65 protein of AR42J was determined by Strept Actividin-Biotin Complex(SABC)method.Results LPS(10 mg/L)resulted in an increase of TNF-αmRNA and TNF-αprotein,whereas significant inhibitory effects were observed when cells were incubated with Tat-NBD(WT)just on dose of 0.1 me/L(P＜0.05).The Tat-NBD(WT)peptide decreased inflammatory cytokine expression by a dose-dependent manner and its peak role was on dose of 100 mg/L.Consisting with TNF-α expression decrease,NF-kB-p65 expression signitieantly decreased in Tat-NBD(WT)pretreatment group,especially on the largest dose.NO significant changes in the control peptide group.Conclusions Tat-NBD(WT)peptide can inhibit the inflammation of acinus simulated by LPS.
