Detection of intracellular calcium concentration and apoptosis in Thapsigargin-induced human colon cell line SW480
- VernacularTitle:SERCA抑制剂抑制人结肠癌SW480细胞生长的作用机制研究
- Author:
Min LU
- Publication Type:Journal Article
- Keywords:
Ca(2+)-transporting ATPase/AI/PD;
Colonic neoplasms/DT
- From:
Journal of Chinese Physician
2009;11(3):334-335
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Thapsigargin in the proliferation of SW480 and provide experimental basis for development of anti-signaling therapy for patients with colon cancer by detection of intracellular calcium concentration in Thapsigargininduced human colon cell line SW480,.Methods Level of[Ca2+]was measured by fluorescence measurement with fura-2/AM,a fluorescent index of calcium.The suppression rate of SW480 cells was calculated by MTY assay.Results Thapsigargin promoted the levels of [Ca2+]from(133.26±5.17,197.46±4.62)to(436.63±5.21,766.41±3.12),and increased the suppression rate from(10.67±5.28)to(31.46±7.21).When W7 Was combined used with Thapsigargin,no significant changes were found in the levels of[Ca2+]and increment of the suppression rate.Condusion Thapsigargin might promote the proliferation of SW480 cells and lead to apoptosis.but the signaling pathway still remain to he studied.
