High metastasis is inhibited in transgenic melanoma cells by endostain gene
- VernacularTitle:转内皮抑素基因抑制黑色素瘤细胞的高转移能力
- Author:
Jianda ZHOU
;
Yuan HU
;
Huiqin XIE
;
Jianxiang TAN
;
Wenbo LI
;
Shaohua WANG
;
Jinyan LIU
- Publication Type:Journal Article
- Keywords:
Endostatins;
Melanoma;
Neoplasm metastasis;
Transfection
- From:
Journal of Chinese Physician
2009;11(3):308-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To validate genetic suppression of metastastic capability of highly metastastic melanoma cells by endostatin transfection.Method pcDNA3.1-Endo eukaryotic expression vector contained insulin signal peptide sequence was transfected into highly metastatic mice melanoma cell strain B 16.The expression of endostain was detected by RT-PCR and Western blot experiment,melanoma cells were determined with adhere experiment,in vitro invasion and migration experiment and pulmonary metastasis experiment on C57BL/6 mice.Result Endostatin can obviously inhibit the capability of adherence,in vitro invasion and migration and pulmonary metastasis of melanoma cells.Among them,adhere inhibition ratio was 67.3%,in vitro invasion inhibition ratio was 48.4%,cell migration inhibition ratiowas 52.1%and pulmonary metastasis inhibition ratio was 67.3%.Conclusion Endostatin transfection can obviously inhibit the highly metastatie capability of melanoma cells.
