X-ray induced TCR gene mutation of peripheral blood lymphocytes

VernacularTitle:X射线诱发外周血淋巴细胞TCR基因突变研究
Author: Dianjun HOU ; Ya MA ; Wei LIU ; Jianwei QIAO ; Feng LU ; Ximei SHANG ; Jieqing LI ; Li FENG
Publication Type:Journal Article
Keywords: Radiation exposure; Gene mutation; TCR gene; Biological dosimeter
From: Chinese Journal of Radiological Medicine and Protection 2009;29(2):151-154
CountryChina
Language:Chinese
Abstract: objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X-ray exposure using cultured lymphocytes cloning method.Methods Freshly isolated peripheral lymphocytes from healthy aduh donors were irradiated with X-ray in doses ranging from 0 to 8 Gy and cultured with interleukin2 and phytohemagglutinin for 7 days.The mutant frequencies of TCR gene(TCR MF)were detected by flow cytonletry and the dose response curves were fitted.Results TCR MF increased with the dose going up.An aquadratic polynomial dose response model was fitted.Conclusions TCR gene mutation could which serve as a potential biological dosimeter.It might be applied for the estimation of biological dose in emergency exposure.