Let-7 and miR-24 in UVB-induced apoptosis

VernacularTitle:Let-7和miR-24在紫外线B诱导的细胞凋亡中的作用
Author: Yingjie HE ; Ling GUO ; Zhenhua DING
Publication Type:Journal Article
Keywords: let-7; miR-24; UVB; Apoptosis
From: Chinese Journal of Radiological Medicine and Protection 2009;29(2):234-236
CountryChina
Language:Chinese
Abstract: Objective To study the function of let-7 and miR-24 in UVB-induced apoptosis.Methods After NIH3T3 cells were irradiated with UVB.Hoechest33342/PI staining was used to study the cell apoptosis and RT-PCR was used to uralyzc the expression level of let-7 and miR-24.In addition,potential target genes of these miRNAs in PicTar were classified into different function categories through GOstat software.Results Compared to the control,the NIH3T3 cells exposure to UVB appeared typical apoptotic and necrotic ceils by fluorescence microscope.The exprossion level of let-7 and miR-24 in NIH3T3 cells after UVB irradiation was higher than that of the control.Among the target genes,casp3,bc1212,map3kl and cdk5 were also involved in UVB-induced apoptosis mechanism.Conclusion Let-7 and miR-24 play a role in UVB-induced apoptosis.