Effects of Dapper1 expression on surviving-mediated cell apoptosis in gastric carcinoma
10.3760/cma.j.issn.1007-631X.2009.04.018
- VernacularTitle:Dapper1在胃癌组织中的表达及其对survivin介导的细胞凋亡的调控
- Author:
Hui QIU
;
Shan WANG
;
Kewei JIANG
;
Yingjiang YE
;
Feng XU
;
Zhirong CUI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Apoptosis;
Cene experssion;
Dapper1
- From:
Chinese Journal of General Surgery
2009;24(4):317-319
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of Dapper1 in gastric carcinoma and elucidate its relationship with survivin and its role in tumor cell apoptosis. Methods Dapper1 mRNA was detected with RT-PCR using specimens from 30 cases of gastric carcinoma and the corresponding normal gastric mucosa.The pcDNA3.1-Dpr1 plasmid was transfected into SGC-7901 cells with LipofectamineTM 2000.The effect of upregulation of Dpr1 on SGC7901 cell apoptosis was determined by flow cytometry.The downregulation of survivin、Dvl-2 and β-catenin protein expression were detected by Western blot analysis.Results Downregulation of Dpr1 gene expression was observed in 17(57%)of 30 human gastric cancer and the downregulation was significantly correlated with the depth of invasion and the degree of differentiation (P＜0.05).Also,upregulation of Dpr1 mRNA and downregulation of survivin mRNA were detected after transfecting pcDNA3.1-Dpr1 plasmid in SGC7901 cells,which led to downregulation of survivin、Dvl-2、β-catenin protein and increase of the SGC7901 cell apoptosis rate from 2.89%to 13.96%.Conclusion Downregulation of Dp1l gene expression is common in human gastric carcinoma,and upregulation of Dpr1 results in significant inhibition of survivin expression which can induce apoptosis of SGC7901 cells.
