The effect of Ras protein on the delayed myocardial protection of Lovastatin preconditioning in the ischemia and reperfused rat hearts
- VernacularTitle:Ras蛋白在Lovastatin预适应心肌保护中的作用及其机制的研究
- Author:
Xiaojie ZHANG
- Publication Type:Journal Article
- Keywords:
Ras proteins;
Lovastatin/TU;
Ischemic preconditioning,myocardial
- From:
Journal of Chinese Physician
2009;11(3):305-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Ras protein on the delayed myocardial protection of Lovastatin preconditioning in the ischemic and reperfused rat hearts.Methods 24 Sprague-Dawley male rats were randomly and evenly divided into three groups,model control group,N group and Lovastatin group.Lovastatin group was treated with 15mg Lovastatin per a kilogram once a day,N group treated with the same dose of Lovastatin with Lovastatin group and L-NAME 30mg per a kilogram once a day.Rat models of I/R were established with coronary occlusion 30 minutes and reperfusion 30 minutes of the left anterior descending artery after two-week's administration.The expression of Ras-GTPase protein in the heart tissue was determined and myocardial apoptosis was detected in all groups.Results The number of TUNEL positive myocardial nuclei in L group was remarkably reduced,compared with C group(t=2.32,P＜0.05).Ras-GTPase protein in the heart tissue was significantly inhibited in the L and N group,compared with C group(t=2.25,P＜0.05).Conclusion Lovastatin may have the delayed protective effect on the ischemie and reperfused rat heart,which effect may be correlated with inhibition of RasGTPase protein during the period of ischemia and reperfusion.Its protective effect was not correlated with NO.
