Roscovitine on cell cycle in mitotic hepatoma carcinoma cell
10.3760/cma.j.issn.1673-4203.2009.04.006
- VernacularTitle:Roscovitine对增生期肝癌细胞周期的影响
- Author:
Jingsheng MA
;
Kaiyang WANG
;
Hongsong XING
;
Huizhen LI
;
Huaqun FU
- Publication Type:Journal Article
- Keywords:
cyclin-dependent kinase;
hepatoma carcinoma cell;
cell cycle;
proliferation;
apeptosis
- From:
International Journal of Surgery
2009;36(4):229-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influences by a Cyelin-dependent kinase inhibitor Roscovitine on cell cycle in mitotic hepatoma carcinoma cell SMMC-7721. Methods Microscope,MTT, flow cytometry and R-T PCR were used to observe the effects of Roscovitine on morphology, proliferation, cell cycle, apoptosis and the mRNA expression of CDK2, Caspase-3, bcl-2 in SMMC-7721 cells. Results Roscovitine inhibited the proliferation of SMMC-7721 cells in dosage and time dependent manner and induced apoptosis. Flow cytometry showed the ratio of G0, G1 increased. R-T PCR showed that the expression of bcl-2 reduced, Caspase-3 increased. Conclusion Reseovitine can inhibit the growth, proliferation, block the cell cycle at G0/G1 and promotes apoptosis of mitotic SMMC-7721 cells, and the mechanism of apoptosis is dependent on the activity of bcl-2 and Caspase-3.
