Detection and analysis of nucleotide sequence of enteroviros 71 from four adults with hand, foot and mouth disease
10.3760/cma.j.issn.1000-6680.2009.03.007
- VernacularTitle:成人手足口病四例患者肠道病毒71型的检测及核苷酸序列分析
- Author:
Weilie CHEN
;
Shaojing WEI
;
Fuchun ZHANG
;
Jian WANG
;
Yangbo TANG
- Publication Type:Journal Article
- Keywords:
Hand,foot and mouth disease;
Enterovirus;
Genotype;
Adult;
Base sequence;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Infectious Diseases
2009;27(3):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the pathogens that cause hand, foot and mouth disease (HFMD) in adults and analyze the nucleotide sequences characteristics of enterovirus 71 (EV71). Methods The reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the enterovirus from the samples of four adult HFMD patients. The 227 bp amplified segments of EVT1 were then sequenced and compared with the sequences of previously isolated EVT1 strains available from GenBank by homogeneity and phylogenetic tree analyses. Results All the results of RT-PCR with enterovirus universal primers and EVT1 specific primers were positive. The EV71 sequences analysis showed that the four new sequences (named as GZ19610, GZ99310, GZ99355 and GZ46477) shared 96.0% to 99.1% nucleotide identify themselves and shared 96.9% to 100.0% homology with the strain Fuyang/17.08/3 isolated in 2008 from Fuyang, Anhui Province. Phylogenetic tree analysis showed that the genotype of the four new sequences was all subtype C4, they were the same sub-genotype as those strains isolated from Chinese mainland and Chinese Taiwan in 2004, and the genetic distance between them was most closely. Conclusions EV71 can cause adult HFMD. Compared with the nucleotide sequences of EV71 strains that isolated now and formerly in China, there is no large variation of the EV71 sequences isolated from four adult HFMD patients in Guangzhou this time. The adult HFMD patients should be isolated for treatment to avoid them transmitting the virus and causing disease spreading.
