Radiation sensitization by CAPE on human HeLa cells of cervical cancer
10.3760/cma.j.issn.0254-5098.2009.02.004
- VernacularTitle:咖啡酸苯一酯对人宫颈癌HeLa细胞的放射增敏作用
- Author:
Xiaoqiang WANG
;
Jianping CAO
;
Saijun FAN
;
Wei ZHU
;
Xiaofei HUANG
;
Yang LIU
;
Xialin CHEN
;
Xiaomei GONG
;
Xiaomei PENG
;
Jing ZENG
- Publication Type:Journal Article
- Keywords:
Caffic acid phenethyl ester;
Radiosensitizing effect;
Cycle checkpoint;
Ionizing radiation
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(2):136-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiosensitizing effect of caffic acid phenethyl ester(CAPE)on human cervical cancer HeLa cells.Methods MTT assay was used to measure the relation between the inhibition effect and CAPE concentrations by CAPE with different concentrations on HeLa cells for 24 hours.HeLa cells were divided into the control and experimental groups,both of which were given 0,2,4,6 and 8 Gy of 60Co γ-irradiation,respectively.The cell clones were counted.Meanwhile HeLa cells were divided into the control,CAPE,irradiation and combination groups.Flow cytometric analysis was adopted to detect the changes of cell cycle distribution induced by CAPE.Results The inhibition rate of CAPE acting on Hela cells increased with concentrations(F=126.49～3654.88,P＜0.01).HeLa cells cloning survival decreased with the increase of radiation dose(F=174.42～9422.81,P＜0.01).At the game radiation dose,HeLa cells cloning survival was less in experimental group than conlrol group(F=120.14～251.91,P＜0.01).The mean lethal dose(D0)(1.45 and 1.82 Gy)and the quasi-threshold dose(Dq)(1.89 and 3.21 Gy)of HeLa cells in experimental group decreased comparing with control group,SER was 1.26.Compared with the sole irradiation group,cells in G2/M phase of the CAPE group and the sole irradiation group increased(P＜0.01)while the combination group decreased(P＜0.01).Conclusions CAPE could increase the radiation sensitivity of HeLa cells by G2/M arrest and may be related to the inhibition of the sub-lethal damage repair.
