Oxidative damage of BEAS-2B cells induced by depleted uranium and protection by DMSO
10.3760/cma.j.issn.0254-5098.2009.02.006
- VernacularTitle:贫铀对BEAS-2B细胞的氧化损伤及二甲基亚砜的保护作用
- Author:
Bo HUANG
;
Feng CHEN
;
Zhihua YANG
;
Xiujie PAN
;
Zhenshan CAO
;
Maoxiang ZHU
- Publication Type:Journal Article
- Keywords:
Depleted uranium;
BEAS-2B cell;
Reactive oxygen species;
Antioxidant enzyme;
DMSO
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(2):143-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the oxidative damage in human bronchial epithelial cells(BEAS-2B) induced by depleted uranium(DU)and protection of DMSO.Methods The measurement of extracellular superoxide anions(O2-·)was based on the reduction of ferricytochrome C.Quantitative analysis of extracellular hydrogen peroxides(H2O2)was used by the horseradish peroxidase-dependent oxidation of phenol red.The determination of extracellular hydroxyl radicals(·OH)was based on discoloration of safranine T.Ethidium bromide and 2,7'-dichlorofluorescein,fluorescent products of the membrane-permeable dyes-hydroethineand 2,7'-dichloroflurescin diacetate were used to monitor the intracellular production of O2-·and H2O2 by fluorometric method.The enzyme activity of SOD and GSH were measured by chemiluminescence and spectrophotometric method,respectively.Results The ROS production,including H2O2,O2-·and·OH,increased remarkably which induced by DU in BEAs-2B cells.The enzyme activity of SOD and GSH was descended remarkedly.These changes could be effectively inhibited by 0.5% of DMSO.Conclusions DU causes oxidative damage to BEAS-2B cells.Through removing active oxygen,DMSO can inhibit oxidative damage of DU.
