Correlation between expression of aquaporin-4 and the apparent diffusion coefficient on diffusion-weighted imaging after focal cerebral ischemia/reperfusion in rats
10.3760/cma.j.issn.1673-4165.2009.03.002
- VernacularTitle:大鼠脑缺血再灌注后水通道蛋白-4表达与弥散加权成像表观弥散系数的相关性研究
- Author:
Hong LU
;
Shizheng ZHANG
;
Hui HU
;
Jian HE
;
Feng ZHAO
;
Tao WU
- Publication Type:Journal Article
- Keywords:
aquaporin-4;
magnetic resonance imaging;
cerebral ischemia;
reperfusion injury;
rats
- From:
International Journal of Cerebrovascular Diseases
2009;17(3):166-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the apparent diffusion coefficient (ADC) and the expression of aquaporin-4 (AQP4) in brain tissue after ischemia/reperfusion in rats. Methods A model of right middle cerebral artery occlusion (MCAO) induced by suture method. Seventy Wistar rats were randomly divided into 7 groups: sham-operation (A), MCAO 30 min (B), MCAO 30 min and reperfusion 30 min (D), MCAO 30 minand reperfusion 60 min (E), MCAO 60 min (C); MCAO 60 min and reperfusion 30 min (F), and MCAO 60 min and reperfusion 60 min (G) groups (n=10 in each group). The rats in all groups underwent diffusion-weighted imaging (DWI). The relative apparent diffusion coefficient (rADC) was calculated. Triphenyltetrazolium chloride (TTC) staining was used to detect the ratio of ischemic area. Immunohistochemistry, in site hybridization, and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expressions of AQP4. Results No abnormal intensity signals were observed on DWI in sham-operation group. The ranges of the high signal intensity lesions on diffusion-weighted imaging (DWI) were from small to large in groups B, C, D, E, F, and G. Then it reduced gradually, so did the ischemic area. The AQP4 expression was up-regulated significantly in groups B and E. The AQP4 expression was decreased significantly after the reperfusion in all groups. 1here was significant difference between ischemic groups and reperfusion groups (all P<0. 05). The expressions of rADC and AQP4 showed negative correlation (r=0. 72, P<0.01). Conclusions The AQP4 expression in brain tissue is closely associated with the changes of ADC after cerebral ischemia/reperfusion. DWI may indirectly reflect the levels of AQP4 expression.
