Effects of the dietary fat on adipose-associated gene expressions of adipocytes in mice
10.3760/cma.j.issn.1000-6699.2009.02.022
- VernacularTitle:不同脂肪含量饮食对小鼠脂肪细胞肥胖相关基因表达的影响
- Author:
Zhong ZHANG
;
Yihong ZHANG
;
Qiang LI
;
Hong QIAO
;
Yuqian SUN
;
Jinchao ZHANG
- Publication Type:Journal Article
- Keywords:
Diet;
Mice;
Adipoeytes;
Gene expression;
Gene chip
- From:
Chinese Journal of Endocrinology and Metabolism
2009;25(2):193-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of dietary fat on diet-induced obesity in mice. Methods Forty 3-week.old male C57BL6 mice were randomly divided into four groups(1-4)fed withdiets of different amounts of lard [0%,20%,40%and 60%of total energy (kcal)].Plasma glucose,lipids,insulin and other parameters were measured after 7 weeks.Moreover,the livers and kidneys were taken for histopathological evaluation and white fatty tissues were taken to analyse adipose'associated geneexpressions using gene chip technology.Results There were statistically significant differences in weight, blood slucose.triglyceride,total cholesterol,high-density lipoprotein-cholesterol,low-density lipoprotein cholesterol.insulin and adiposity index between the 1st and the 4th group.Obvious lipid deposition in the liver and kidney sections was found in the 4th group.Thirteen adipose-associated genes were up-regulated in exDression and 8 genes down.regulated by over 2-fold in the adipocytes of the 4th group. Conclusions The mice fed with high-fat diet developed evident obesity,insulin resistance and abnormal lipid metabolism.The obesity was induced by high-fat diet via regulating some of the orexigenic genes,anorexigenie genes and the genes involved in energy expenditure in adipocytes of mice.
