Genetic analysis of chorionic villi specimen in spontaneous abortion using various methods
10.3760/cma.j.issn.0529-567x.2009.04.004
- VernacularTitle:不同分子遗传学方法用于自然流产绒毛细胞遗传分析的效果
- Author:
Qiong LEI
;
Qiong WANG
;
Canquan ZHOU
;
Baojiang CHEN
;
Zheng CHEN
;
Yanwen XU
;
Yanhong ZENG
- Publication Type:Journal Article
- Keywords:
Abortion,spontaneous;
Chorionie villi;
Nucleic acid probes;
Gene amplification;
Nucleic acid hybridization;
In situ hybridization,fluorescence
- From:
Chinese Journal of Obstetrics and Gynecology
2009;44(4):253-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the efficacy of multiplex ligation-dependent probe amplification (MLPA)combined with fluorescence in situ hybridization(FISH)and comparative genomie hybridization (CGH)combined with FISH in genetic analysis of chorionic villi specimen(CVS)of spontaneous abortion.Methods CGH+FISH and MLPA+FISH were used for genetic analysis of 29 CVS from spontaneous abortion and 6 normal CVS from selective abortion,in the mean time,those results were compared with conventional eytogenetic karyotyping.Results The report time were 40 hours in MLPA+FISH and 120 houm in CGH+FISH.The mean time of chorionic villi culture was(240±72)hours.The successful rate of specimen analysis were 97%(34/35)in CGH,100%(35/35)in MLPA,100%(35/35)in FISH and 91%(32/35)in conventional cytogenetic karyotyping.Apart from 1 case failed in CGH analysis,the results from MLPA+FISH were almost similar to that from CGH+FISH,however,that 1 specimen failed in CGH were detected successfully by MLPA+FISH.The discrepancy rate were 13%(4/31)in CGH+FISH and 12%(4/32)in MLPA+FISH respectively when compared with conventional cytogenetic analysis.Conclusions MLPA+FISH analysis present shorter detecting time and achieve 100%tale of successful report.This combined method was an important adjuvant approach to conventional cytogenetic karyotyping in CVS from spontaneous abortion.
