Effects of Ginkgo biloba extract EGb761 on the expressions of XIAP and Smac following focal cerebral ischemia/reperfusion in rats
10.3760/cma.j.issn.1673-4165.2009.03.001
- VernacularTitle:银杏叶提取物EGb761对局灶性脑缺血再灌注大鼠XIAP和Smac表达的影响
- Author:
Limei CAO
;
Guinan BI
;
Chuanming LUO
;
Xu CHEN
- Publication Type:Journal Article
- Keywords:
Ginkgo biloba;
XIAP;
Smac;
cerebral ischemia
- From:
International Journal of Cerebrovascular Diseases
2009;17(3):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ginkgo biloba extract EGb761 on the expressions of XIAP and Smac following focal cerebral ischemia in rats. Methods A total of 40 male Wistar rats were randomized into 4 groups: sham-operation group, cerebral ischemi-a/reperfusion group, low-dose EGb761 group, and high-dose EGb761 group (n=10 in each group). A rat model of middle cerebral artery occlusion (MCAO) for 1.5 hours and reperfusion for 24 hours was built. EGb761 50 mg/kg and 100 mg/kg were injected intraperitoneally at one hour before the model building in the low-dose EGb761 and high-dose EGb761 groups. The expressions of XIAP and Smac in brain tissue were detected by immunohistochemistry method. Results The expressions of XIAP were 18. 33±4. 01 and 26. 7±3.27 respectively in the low-dose EGb761 and high-dose EGb761 groups, and they were significantly higher than 12. 13±3.44 in the cerebral ischemia/reperfusion group (all P<0.01), and the high-dose EGb761group was higher than the low-dose EGb761 group (P<0.01). 1he expressions of Smac in brain tissue were 21.33±3.15 and 11.33±2. 10 respectively in the low-dose EGb761 and high-dose EGb761 groups, and they were significantly lower than 28.93±4. 96 in the cerebral ischemia/reperfusion group (all P<0.05). The high-dose EGb761 group was significantly lower than the low-dose EGb761 group (P<0.05). Conclusions Cerebral ischemia/reperfusion could induce the expressions of XIAP and Smac. EGb761 intervention could inhibit the expressions of Smac while upregulating the expression of XIAP, and increase the XIAP/Smac ratio. 1his may be one of the protective mechanisms of EGb761 intervention.
