Analysis and perform human sperm vitality staining techniques
- Author:
Tran Duc Phan
- Publication Type:Journal Article
- Keywords:
Semen;
Infertility;
Vitality;
Eosin;
Trypan blue
- MeSH:
Spermatozoa, Human
- From:Journal of Medical Research
2005;39(6):25-30
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Infertility is a common health problem. For investigating the reason of infertility in the man, semen analysis plays very important role. Vitality stain is one of semen analysis. It is rare use for diagnosing and evaluating the result of infertility treatment. Our objectives: (1) To submit a fully worked-out of vitality staining technique. (2) To compare the effects of eosin and trypan blue staining for measuring live and death sperms. Methods: 52 semen samples were analyzed, compared the rate of death sperm base on the time after staining by eosin and trypan blue. Results: The average time for staining sperms by eosin: 16 seconds, by trypan blue: 19.67 seconds. The best process are: Mix 1 volume of eosin and 4-5 volume of semen, 1 volume of trypan blue and 2 volume of semen. Conclusions: (1) The more staining time, the more number of death sperm. (2) In vitality staining technique: The volume of semen should must be more than volume of stain chemical.
