Differential expressions of α-defensin between chronic hepatitis B and asymptomatic HBV carriers
10.3760/cma.j.issn.1674-2397.2009.02.007
- VernacularTitle:α防御素在慢性乙型肝炎患者和HBV携带者之间的差异表达
- Author:
Cuilan TANG
;
Zhi CHEN
- Publication Type:Journal Article
- Keywords:
Hepatitis virus B;
Chronic hepatitis B;
Asymptomatie HBV carriers;
α-defensin
- From:
Chinese Journal of Clinical Infectious Diseases
2009;2(2):86-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore different pathogenesis of chronic hepatitis B(CHB)and asymptomatic H BV carriers(ASCs)by identifying differentially expressed genes.Methods Subtracted library was constructed by suppression subtraetive hybridization(SSH),and α-defensin was identified by dot blot hybridization.Peripheral blood was collected from 46 CHB patients and 11 ASCs.and the expressions of α-defensin mRNA in peripheral blood mononuclear cell and protein in plasma were determined by the real time RT-PCR and enzyme-linked immunosorbent assay(ELISA).Results Real time RT-PCR showed that the expression of α-defensin mRNA in blood samples of CHB was 1.4-fold higher than that of ASCs.As shown by ELISA,the plasma level of α-defensin in CHB was higher than that of ASCs [(216.40±81.25)μg/L vs.(156.00±57.26)μg/L,t=2.23,P<0.05].Conclusion α-defensin may involve in the pathogenesis of CHB,for it iS over-expressed in CHB patients.
