Effects of erythropoietin pretreatment on acute lung injury induced by lipopolysaccharide in rats
10.3760/cma.j.issn.0254-1416.2009.04.018
- VernacularTitle:促红细胞生成素预先给药对大鼠内毒素性急性肺损伤的影响
- Author:
You SHANG
;
Xingwang LI
;
Dong LIU
;
Dan FENG
;
Shanglong YAO
;
Shiying YUAN
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Endotoxemia;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2009;29(4):349-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of erythropoietin (EPO) pretreatment on the acute lung injury induced by lipopolysaccharide (LPS) in rats and the underlying mechanism. Methods Thirty-two male SD rats weighing 180-220 g were randomly divided into 4 groups (n=8 each): group Ⅰ control (C);group Ⅱ EPO;group Ⅲ LPS and group Ⅳ EPO + LPS. EPO 3 000 U/kg was given IP in group Ⅱ , LPS 6 mg/kg was given iv in group Ⅲ . In group Ⅳ EPO 3 000 U/kg was given IP at 30 rain before iv LPS 6 mg/kg, The animals were killed at 4 h after LPS administration. Lung tissue specimens were obtained for microscopic examination. Wet/dry ratio (W/D), myeloperoxidase (MPO) activity, malondialdehyde (MDA) and nitric oxide (NO) content in lung tissue were determined. The expression of inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT) in lung tissue was determined by Western blot. Results W/D ratio, MPO activity, MDA and NO content were significantly increased and iNOS and NT expression was significantly up-regulated in LPS group as compared with control group. EPO pretreatment significantly attenuated the LPS-induced changes in group EPO + LPS. Conclusion EPO pretreatment can ameliorate the acute lung injury induced by LPS by down-regulating iNOS expression and reducing NO production.
