Docetaxel induced human uterine cervical carcinoma cell apoptosis and it's enhancement effect on radiosensitization
- VernacularTitle:多西紫杉醇诱导人宫颈癌细胞凋亡对其放射增强作用的影响
- Author:
Liping TANG
;
Rong MA
;
Xiaoxing GENG
;
Yanmei YANG
- Publication Type:Journal Article
- Keywords:
Paelitaxel/PD;
Cervix neoplasms;
Apoptosis;
Radiation tolerance/DE
- From:
Journal of Chinese Physician
2009;11(4):468-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate therapeutic mechanism of radiation therapy associated with Doeetaxel on human uterine cervi-cal carcinoma. Method Hela cells were incubated with 0. 5 μmol/L, 1.01 μmol/Docetaxel for 24 hours, and were radiated with 0, 2, 4, 6, 8Gy X ray. The proliferation activity was detected with MTT method. Apoptosis was evaluated with TUNEL method. Expression of caspase-3 protein and the degradation fragment was measured with Western blot. Result Inhibitory effect of radiation therapy associated Docetaxel on Hela cells was significantly stronger than that of radiation group. Treated with 2 Gy and 6MV X ray radiation, inhibitory rate of Hela cells increased with the increment of associated Docetaxel doses (P<0.05). Inhibitory rate of Hela cells in radiation associated Docetaxel group was significantly increased compared with radiation group. Apoptosis index in radiation associated Docetaxel group 48 hours after treatment was significantly increased compared with radiation group or Docetaxel group with TUNEL method (P<0.05). Western blot results indies-ted that caspase-3 protein was degraded and small active fragments were produced. Conclusion The reason that Docetaxel enhanced Hela cell radiation sensitivity may probably he correlated to easpase-3 protein activation.
