Effects of endotoxin on NF-κB mRNA expression and aldosteron secretion in rat hepatic stellate cells

VernacularTitle:内毒素对大鼠肝星状细胞NF-κB mRNA表达及醛固酮分泌的影响
Author: Lili HUANG ; Longfeng ZHAO ; Yongqing DOU ; Hong LI ; Yanqin HAO
Publication Type:Journal Article
Keywords: Endotoxins; Aldosteron; Hepatic stellate cell; Liver fibrosis; Nuclear factor-κB p65
From: Chinese Journal of Clinical Infectious Diseases 2009;2(2):82-85
CountryChina
Language:Chinese
Abstract: Objecfive To investigate the effects of endotoxin on nuclear factor-κB p65(NF-κB p65)mRNA expression and ahtosteron secretion in rat hepatic stellate cells(HSCs).Methods Cultured rat HSCs(HSC-T6)were divided into endotoxin-treated group and control group.Cells in endotoxin-treated group were exposure to 1 mg/ml.endotoxin.Aldosteron secretions of HSCs were determined by radioimmunoassay,and NF-κB p65 mRNA expressions of HSCs were detected by one-step RT-PCR.Results At 6,12,24 and 48 h,aldosteron secretions in endotoxin-treated group were significantly hisher than those in the control group(t=3.063,4.577,6.847 and 9.317,P<0.05),and the expressions of NF-κB p65 mRNA in endotoxin-treated group were also higher than those in control group(t=5.155,6.095,7.875 and 9.313,P<0.01).Aldosteron secretions and NF-κB p65 mRNA expressions in HSCs displayed a positive correlation(r=0.886,P<0.01).Conclusion Endotoxin can up-regulate the aldosteron secretion and NF-κB p65 mRNA expression in rat HSCs,which may be one of the mechanisms of liver fibrosis induced by endotoxin.