Reduced population of CD4+,CD154+ T cell subset in patients with active pulmonary tuberculosis
- VernacularTitle:活动期肺结核患者CD4+、CD154+T细胞亚群数量的改变
- Author:
Xinjing WANG
;
Xiaoxing CHENG
;
Zhihong CAO
;
Yan ZHU
;
Mei DONG
;
Aihua TONG
- Publication Type:Journal Article
- Keywords:
Tuberculosis,pulmonary;
CD4-positive T-lymphocytes;
CD40 ligand;
T-lympheytes
- From:
Journal of Chinese Physician
2009;11(4):433-435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study population frequencies of CD4+,CD154+ T cell subset in patients with pulmonary tuberculosis and controls with positive PPD reaction. Methods Flow cytometry was used to detect the CD4+,CD154+ T cell subset, the population frequen-cies in patients with pulmonary tuberculosis and controls were compared. Results The expression level of CD154 was higher when PE-la-beled CD154 antibody was added during stimulation period, compared with CD154 labeling after stimulation(1.51±0. 36/0. 40±0. 13, P <0.05). The CD154+ cells were not detectable in fresh isolated CD4+ T cells, but significantly increased after stimulation with specific anti-gens. The population of CD4+, CD154+ T cell subset was significantly reduced in patients with active pulmonary tuberculosis, compared with healthy controls with PPD positive reaction(0. 72±0. 32/1.65±0. 76, P <0. 01). Conclusions The population of CD4± ,CD154± T cell subset was significantly reduced in patients with active pulmonary tuberculosis, which indicated that it may play an important role in the de-velopment of tuberculosis.
