The role of endoscopy iodine staining combined detecting the expression of p53 ,survivin and telomerase in the diagnosis of early e-sophageal carcinoma and precancerom lesions
- VernacularTitle:碘染色联合p53、Survivin及端粒酶检测对早期食管癌及癌前病变的诊断价值
- Author:
Chunan LIU
;
Xindong ZHANG
;
Yan YANG
;
Kai MA
;
Jinzhi BAI
;
Xiangge WANG
;
Jin WANG
;
Tonghai CHAI
- Publication Type:Journal Article
- Keywords:
Iodine componnds/DU;
Staining and labeling;
Genes,p53;
Microtubule-associated proteins;
Telomerase;
Esophageal neoplasms/DI
- From:
Journal of Chinese Physician
2009;11(4):447-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the value of using endoscopy iodine staining combined detecting the expression of p53, survivin and telomerase in the diagnosis of early esophageal carcinoma and precancerous lesions. Methods Three hundred patients who received routine gastrescopy and had high risk of esophageal carcinoma were examined by the iodine dyeing on their esophageal mucosa with 2. 5% Lugol solu-tion. Multiple biopsies were taken from the unstained or light-stained areas when their diameters were over 5mm for pathology diagnosis. The expression of p53, survivin and telomerase were detected by immunohistochemical staining. Results With the Lugol staining method, the incidences of light, moderate and high grade dysplasia and early esophageal carcinoma were 16. 3% ,9. 7% ,3.7% and 4. 3% respectively. The expression of p53, survivin and telomerase were related and the unstained were related to the expression of p53, survivin and telomerase in dysplasia and early esophageal carcinoma. Using endoscopy iodine staining combined testing p53, survivin and telomerase showed sensitiv-ity of 100%, specificity of 92. 3% and accuracy of 91.8% to diagnose of early esophageal carcinoma and high grade dysplasia. Conclusion Lugol solution chromoendscopy combined assay of p53, survivin and telomersse might be useful in the diagnosing early esophageal carcino-ma and precancerous lesion.
