Effect of angiotensin-(1-7) on proliferation and secretion of cultured rat's glomerular mesangial cells induced by transforming growth factor-β1
- VernacularTitle:血管紧张素-(1-7)对转化生长因子-β1诱导系膜细胞增殖及细胞外基质分泌的影响
- Author:
Hongyan HE
;
Jun LI
;
Jian LIU
;
Mingyong WANG
- Publication Type:Journal Article
- Keywords:
Angiotensins;
Transforming growth factor beta;
Glomerular mesangium;
Cell proliferation;
Extracellular matrix
- From:
Journal of Chinese Physician
2009;11(4):450-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of angiotensin-(1-7) [Ang-(1-7)] on proliferation and secretion in cultured rat's glo-merular mesangial cells(GMC) induced by transforming growth factor-β1 (TGF-β1). Methods GMC in logarithmic growth phase were in-cubated, and then were divided into 4 groups: control, Ang-(1-7), TGF-β1,and TGF-β1 + Ang-(1-7) group. Cell numbers of rat's GMC were detected by WST-1. Laminin(LN) and collagenlV (ColⅣ) mRNA expressions in GMC were analyzed by RT-PCR. The secretion of LN and ColⅣ in culture medium of rat's GMC was measured by radioimmunoassay. Results Ang-(1-7) significantly inhibited basal and TGF-β1-induced proliferation of cultured glomerular mesangial cells. Ang-(1-7) significantly down-regulated LN and Col Ⅳ mRNA expressions in glomerular mesangial cells and also inhibited the secretion of LN and ColⅣ induced by TGF-β1(P <0. 05). Conclusions Ang-(1-7) can inhibit basal and TGF-β1-induced proliferation, and inhibit ECM secretion of cultured rats glomerular mesangial cells.
