The effects of acetylcysteine on proliferation and collagen synthesis of human lung fibroblast
10.3760/cma.j.issn.1008-6315.2009.05.005
- VernacularTitle:乙酰半胱氨酸对人肺成纤维细胞增殖及胶原合成的影响
- Author:
Jianming HU
;
Xiaohong CHEN
;
Nanshan ZHONG
- Publication Type:Journal Article
- Keywords:
Acetylcysteine;
Lung fibroblasts;
Proliferation;
Apoptosis;
Collagen
- From:
Clinical Medicine of China
2009;25(5):459-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of N-acetylcysteine (NAC) on the proliferation, apoptosis and collagen synthesis of human lung fibroblast (HLF). Methods HLF was primarily cultured in complete medium of DMEM/F12. Different concentrations of NAC (5,10,20,40mmol/L) were administrated. Cell proliferations were tested by methylthiazolyltetrazolium (MTT) ,apoptosis and cell cycle were detected with Flow cytometer and mRNA and 40 mmoi/L )after 24 hours,the apoptosis rates were (34.38±5.80)%, (37.72±3.10)%, (44.05± 4.52) % and (59.18±5.24) % ) respectively, significantly higher than that of the controls (3.92±1.24) % pression of type Ⅰ procollagen in HLF was decreased significantly after administration of NAC at 5, 10,20, 40 mmol/L respectively (P < 0.01 ). Conclusion Administration of NAC induces apoptosis and directly inhibites the proliferation and the collagen synthesis of HLF.
