Fetal liver stem cells for the treatment of murine liver injury

VernacularTitle:胚胎肝干细胞分离与移植治疗裸鼠急性肝损伤
Author: Qiangfeng YU ; Jianyin ZHOU ; Zhenyu YIN ; Wenxiu ZHAO ; Xiaomin WANG
Publication Type:Journal Article
Keywords: Liver failure,acute; Stem cell transplantation; Embryonic stem celia; Animal experimentation
From: Chinese Journal of General Surgery 2009;24(5):390-393
CountryChina
Language:Chinese
Abstract: Objective To isolate and indentify fetal hepatic progenitor/stem cells, and study the feasibility and effectiveness of their transplantation on acute liver injury in nude mice. Methods The primitive cells isolated from 13.5dpc pregnant mouse fetal hver by way of enzyme digesting were cultured in vitro and liver specific markers as AFP, CK19, Albumin, c-Met, were identified by immunofluorescence and RT-PCR on colonies. 4 × 105 cells were transplanted into nude mice with carbon tetrachloride induced acute liver injury. Hapatic functions were measured pre- and -post transplantation on days 1,2,4, 7. Meanwhile, hepatic pathology was studied. Results Compared to control group the hepatic functions gradually recovered in transplant group, on days 1,2,4 after fetal hepatic stem cell transplantation. The hepatic pathology significantly improved in stem cells transplantation group. Conclution Fetal hepatic progenitor/stem cell were successfully yielded by enzyme digest. Stem cells transplantation improved the hepatic function and pathology in acute hapatic injury model of nude mice.