Experimental studies of the effect of erythropoietin on fas-associated death domain protein and caslmse-8 protein in rat with intracerebral hemorrhage
10.3760/cma.j.issn.1008-6315.2009.05.004
- VernacularTitle:促红细胞生成素对脑出血大鼠脑血肿周围Fas相关死亡域蛋白和Caspase-8表达的影响
- Author:
Qiuyan SHI
;
Jinke JIANG
;
Qian LI
;
Chao LIU
;
Huifang SUN
;
Junfang HE
;
Guozhi ZHANG
;
Ruibiao ZHANG
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Intracerebral hemorrhage;
Fas-associated with death domain protein;
Caspase-8
- From:
Clinical Medicine of China
2009;25(5):456-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein expressions of Fas-associated death domain protein (FADD) and caspase-8 in rats with intracerebral hemorrhage ,and the effects of erythropoietin tp reveal the mechanism of neu-m-protection by EPO. Methods 126 male SD rats were randomly divided into three groups: Sham-operated group, intracerebral hemorrhage group, and EPO group. Each group was divided into seven subgroups according to the differ-ent time points (3,6,12,24,48,72 h and 7 d). The model of intracerebral hemorrage was established in rats by in-tracerebral injection of autogenous blood. The protein expressions of FADD and caspas-8 in rats tissue around the hemorrhagic and the normal brain tissue were detected by immunohistochemistry. Results The protein expressions of FADD and caspase-8 were increased [(4.66±0.46 ) and ( 15.89±1.81)] at 3 h after intracerebral hemorrhage, and peaked at 48 h [ (35.88±4.24 ) and (45.04±3.99)], the expressions of FADD and caspas-8 in the region around hematoma in EPO group significantly decreased compared with model group[ (3.92±0.64) and (28.24±1.90), (13.32±2.01 ) and (35.08±2.82)] at 3 h and 48 h. Conclusion The protein expressions of FADD and easpase-8 are markedly increased after intracerebral hemorrhage. EPO can protect the neurons by signifi-cantly reducing the expressions of FADD and caspase-8.
