Effect of keratinocyte growth factor receptor transgene on sodium channel in alveolar type Ⅱcells with LPS-induced acute hmg injury
10.3760/cma.j.issn.1671-0282.2009.05.002
- VernacularTitle:角质细胞生长因子受体转基因对急性肺损伤时肺泡上皮细胞钠离子通道的影响
- Author:
Binjian LIU
;
Ghaoling QI
;
Shuhui ZHENG
;
Muxiu ZHOU
;
Jianming WANG
- Publication Type:Journal Article
- Keywords:
Keratinocyte growth factor receptor;
Sodium ehannol;
Acute lung injury;
Gem therapy
- From:
Chinese Journal of Emergency Medicine
2009;18(5):457-461
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of keratinocyte growth factor receptor (KGFR) transgene on sodium channel in alveolar type Ⅱ cells with LPS-induced acute lung injury, to provide the evidence for gene treatment in acute lung injury. Method Totally 40 male Sprague-Dawtey rats were randomly divided into four groups, including normal control (n=8), injured control (n=10), normal transgene (n=10) and injured transgene (n=12). The models of acute lung injury were produced using LPS, and the successful criteria was the obvious enlargement in the lung tissue. The rats in normal transgene group and injured transgene group were injected with 1 mL of KGFR adenovirus vector through rats' tail vein. At 72 hours later, the rats in injured control group and injured transgene group were injected with LPS in dose of 5 mg/kg (BW). While rats in normal control group and normal transgene group were injected with equivalent saline simultaneously. Another 48 hours later, rats in the four groups were killed. The lung tissue were collected for analysis. The expression of sodium channel in rats' alveolar type Ⅱ cells were detected by immunohistochemistry and immunoeectron microscope. Difference among the experimental groups were estimated by ANOVA analysis (LSD-t-test). There was statistical signifi-cance when P<0.05. Results The levels of sodium channel expression in rats' alveolar type Ⅱ cells were differ-era, with normal control group (47.7±3.33), normal transgene group (46.9±5.21), injured tramgene group (29.19±4.11) and injured control group (5.1±2.3). The level of sodium channel expression in injured trans-gene group was lower than that in normal transgene group (t=9.134, P<0.001) and normal control group (t=10.601,P<0.001), but signifieantly higher than that in injured control group (t=16.466, P<0.001). Conclusions The transgene vector can effectively promote the expression of sodium channel in alveolar type Ⅱ cells in rats with LPS-indueed acute lung injury, and can alleviate sodium and water reteraion in alveolar.
