Experimental study of the effects of olfactory lamina propria transplantation and ganglioside GMI treatment on spinal curd injury
10.3760/cma.j.issn.0254-9026.2009.05.018
- VernacularTitle:鼻嗅黏膜固有层移植及神经节苷脂治疗脊髓损伤的实验研究
- Author:
Zhonghao LIU
;
Mingzhi GONG
;
Shiqing WU
;
Deguo XING
;
Ruoyi WANG
- Publication Type:Journal Article
- Keywords:
Olfactory mucosa;
G(M1) ganglioside;
Tissue transplantation;
Spinal cord injuries
- From:
Chinese Journal of Geriatrics
2009;28(5):418-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment on spinal cord injury (SCI) in rats.Methods Totally 50 healthy pure breed female Sprague-Dawley (SD) rats after spinal cord hemiseetion were randomly divided into 5 groups and were given different treatments: (OLP + GM1) treatment group (group A), GM1 treatment group (group B), OLP treatment group (group C), spinal cord injury but without treatment group (group D) and healthy control group (group E). The recovery of neurological function was evaluated by somatosensory evoked potential (SEP) and pathological examination after surgery. Results In group A, in some rats an escaping response in right hind leg occurred, but in other groups, the motor function was not significantly improved. Histological examination showed that transplanted olfactory lamina propria survived in the transplantation area and expanded on certain routes. NF positive nerve fibers passed through the transplantation area. Compared with group B, C, D, the N1-wave latency was(4.71±0. 72)ms 4 weeks after operation(P<0. 01), and the NF density was(7. 31±0. 26) ×104/mm28 weeks after operation in group A(P<0. 05). Conclusions Olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment have a synergistic effect on SCI.
