Effects of alendronate on adipogenic differentiation of bone marrow stromal cells derived from ovariectomized rats
10.3760/cma.j.issn.1001-8050.2009.02.53
- VernacularTitle:阿伦膦酸钠对去势大鼠骨髓间充质干细胞成脂分化的影响
- Author:
Lingjie FU
;
Tingting TANG
;
Kerong DAI
- Publication Type:Journal Article
- Keywords:
Alendronate;
Bone marrow stromal cells;
Mitogen-activated protein kinases;
Osteoporosis
- From:
Chinese Journal of Trauma
2009;25(2):177-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of alendronate on adipogenic differentiation of bone marrow stremal cells (BMSCs) and the role of mitogen-activated protein kinases (MAPK) signal pathway in this process. Methods BMSCs were derived from 9-month-old ovariectomized SD rats and exposed to 0.01, 0.1, 1, 10 μmol/L of alendronate for 2 weeks. The number of BMSCs was counted under light microscope after oil red O staining. The expression of peroxisome proliferators activated receptor-γ, 2 (PPAR-γ2) was measured by RT-PCR. The effect of alendronate on MAPK signal pathway was detected by Western blot. Results After two weeks of induction of BMSCs by alendronate, BMSCs with positive oil red O staining significantly decreased as the increase of the concentration of alendronate (P <0.01), so did the expression of PPAR-γ2. The expression level of PPAR 2 increased when exposing BMSCs to ERK1/2 or JNK specific inhibitors, PD98059 and SP600125 for two weeks. However, the expression level of PPAR 2 decreased when exposing BMSCs to SB203580 (an inhibitor of p38) for two weeks. Condusion Alendronate can inhibit adipogenic differentiation of BMSCs derived from ovariectomized rats in a doso-dependent manner by activating ERK1/2 and JNK rather than p38.
