Comparison of the effect of glucosamine on the cartilage oligomeric matrix protein secretion in vitro chondrocytes and synoviocytes
10.3760/cma.j.issn.1007-7480.2009.05.014
- VernacularTitle:氨基葡萄糖对体外培养人软骨和滑膜细胞软骨寡聚基质蛋白分泌影响的比较
- Author:
Yuxin ZHENG
;
Yuelong CAO
;
Guantong SHI
;
Dapeng HAN
;
Peng ZHANG
;
Dengxiao LI
;
Hongsheng ZHAN
;
Yinyu SHI
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Cartilage;
Synovial membrane;
Extracellular matrix peoteins
- From:
Chinese Journal of Rheumatology
2009;13(5):331-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of glucosamine (Virtral-s) on the cartilage oligomeric matrix protein (COMP) secretion of chondrocytes and synoviocytes in vitro.Methods Chondrocytes and synoviocytes isolated from knee cartilage of osteoarthritic patients were cultured by phased enzymatic digestion.Sera containing Virtral-s of the experimental animals were obtained after orally administrated Virtral-s at the dosages that equal to human.Cells were cultured in the medium with Virtral-s containing sera.Super-natant COMP level was tested by enzyme-linked immunoabsorbent assays (ELISA).Results COMP conceu-tration of synoviocytes cultured in vitro was significantly higher than that of chondrocytes (P＜0.05).Virtral-s could significantly increase COMP secretion in cultured chondrocytes in vitro (P＜0.05),however,it had a weaker role on synoviocytes,ie,it could only mildly reduce COMP secretion of synoviocytes.Conclusion Glucosamine (Virtral-s)-containing serum can promote COMP secretion of chondrocytes in vitro,and it has no significant effect on synoviocytes in vitro.
